Carotenoids are bioactive molecules known to promote human health. Many extreme halophilic archaea
synthesize carotenoids, mainly represented by C50 bacterioruberin (BR) and its derivatives. BR has a potent
antioxidant capacity, even higher than that of β-carotene, thus, there is an increasing interest to advance the
study of its biological properties as well as to extend its current applications. Here, we describe a procedure
to extract and characterize carotenoids (enriched in BR) from haloarchaea using a “hyperpigmented”
genetically modified strain of Haloferax volcanii.
Churio, M.S., Cerletti, M., De Castro, R.E. (2022). Carotenoids from Haloarchaea: Extraction, Fractionation, and Characterization. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_21
Link to chapter: https://link.springer.com/protocol/10.1007/978-1-0716-2445-6_21