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Many research areas, e.g., basic research but also applied fields of biotechnology, biomedicine, and
diagnostics often suffer from the unavailability of metabolic compounds. This is mostly due to missing
easy and efficient synthesis procedures. We herein describe the biocatalytic/enzymatic production of
2-keto-3-deoxy-D-gluconate, an intermediate of central metabolic pathways in all three domains of life
and also of bacterial polysaccharides, lipopolysaccharides, and cell wall components. The method is based
This protocol describes how to synchronise a S. acidocaldarius cell culture. The first step is to administer acetic acid to arrest the cells in a G2-like state. Once washed, the culture proceeds synchronously through division and DNA replication for about two cell cycles.
Patro, M., van Wolferen, M., Ye, X., Albers, SV., Quax, T.E.F. (2022). Methods to Analyze Motility in Eury- and Crenarchaea. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_25
Compiled and edited by Dr Mike Dyall-Smith
Sulfolobus acidocaldarius is one of few genetically tractable archaeal species. This protocol outlines how to transform S. acidocaldarius with electroporation.
