Methods to Analyze Motility in Eury- and Crenarchaea

Authors
Dr. Megha Patro, Dr. Marleen van Wolferen, Dr. Xing Ye, Dr. Sonja V. Albers, Dr. Tessa E.F. Quax
Phylogeny
Euryarchaeota
TACK group
Thermoproteota
Sulfolobales
Expertise
Microscopy
Motility assay

Patro, M., van Wolferen, M., Ye, X., Albers, SV., Quax, T.E.F. (2022). Methods to Analyze Motility in Eury- and Crenarchaea. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_25

Link to chapter: https://link.springer.com/protocol/10.1007/978-1-0716-2445-6_25

Abstract
Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeon Haloferax volcanii and the crenarchaeon Sulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.