Zenodo DOI
10.5281/zenodo.11475196
Phylogeny
TACK group
Thermoproteota
Sulfolobales
Sulfolobus acidocaldarius
Reviewers
Group leader
Keywords
Synchronisation
Cell cycle
Editor
This protocol describes how to synchronise a S. acidocaldarius cell culture. The first step is to administer acetic acid to arrest the cells in a G2-like state. Once washed, the culture proceeds synchronously through division and DNA replication for about two cell cycles.
Related publication(s): Tarrason Risa et al, 2020 (this version) and Lundgren et al., 2004 (original version)