Phylogeny
TACK group
Sulfolobales
Sulfolobus acidocaldarius
Sulfolobus islandicus
Institution
Group leader
Keywords
EdU staining
Cell cycle
Fluorescence Microscopy
Flow Cytometry
Expertise
Cellular Biology
Cells synchronization
Microscopy
Editor
This protocol describes how to stain EdU after its incorporation into Sulfolobus acidocaldarius. EdU incorporation allows visualization of newly synthesized DNA, making it a useful method for studying the cell cycle. The staining process involves an EdU Click-It reaction using Alexa Fluor azide and subsequent washing steps before analysis by microscopy or flow cytometry. Compared to other DNA labeling techniques, this protocol offers high specificity and ease of implementation.