Haloferax cloning

The Magic Book has been developed by the Allers Lab, detailing experimental procedures for working with Haloferax volcanii, a model archaeon. The protocol book includes standardized materials and methods covering a wide range of molecular biology and genetic techniques specific to H. volcanii. 

This protocol outlines the molecular biology techniques such as PCR amplification, restriction digestion, dephosphorylation, blunt-end cloning, ligation, agarose gel electrophoresis, and plasmid cloning.