Phylogeny
Euryarchaeota
Stenosarchaea group
Halobacteria
Haloferax volcanii
Institution
Group leader
Keywords
Genetic Manipulation
Gene deletion
Pop-in and Pop-out
Expertise
Genetic manipulation
The Magic Book has been developed by the Allers Lab, detailing experimental procedures for working with Haloferax volcanii, a model archaeon. The protocol book includes standardized materials and methods covering a wide range of molecular biology and genetic techniques specific to H. volcanii.
This protocol describes the genetic manipulation of Haloferax using the pop-in/pop-out method to generate targeted gene deletions or modifications.
Additional related protocols can be found separately on the website under the following titles:
- Haloferax Media (general media preparation)
- Haloferax: Genomic DNA Miniprep
- Haloferax Transformation
- Haloferax Southern Blot