Genetic manipulation is an essential tool to investigate complex microbiological phenomena. In this
chapter we describe the techniques required to transform the model hyperthermophilic, anaerobic
archaeon Thermococcus kodakarensis. T. kodakarensis can support two modes of genetic manipulation,
dependent either on homologous recombination into the genome or through retention of autonomously
replicating plasmids. The robust genetic system developed in T. kodakarensis offers a variety of selectable
and counterselectable markers for complex, accurate and iterative genetic manipulations offering greater
flexibility to probe gene function in vivo.
Liman, G.L.S., Stettler, M.E., Santangelo, T.J. (2022). Transformation Techniques for the Anaerobic Hyperthermophile Thermococcus kodakarensis. In: Ferreira-Cerca, S. (eds) Archaea. Methods in Molecular Biology, vol 2522. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2445-6_5
Link to chapter: https://link.springer.com/protocol/10.1007/978-1-0716-2445-6_5