EdU staining protocol for Sulofolobus

This protocol describes how to stain EdU after its incorporation into Sulfolobus acidocaldarius. EdU incorporation allows visualization of newly synthesized DNA, making it a useful method for studying the cell cycle. The staining process involves an EdU Click-It reaction using Alexa Fluor azide and subsequent washing steps before analysis by microscopy or flow cytometry. Compared to other DNA labeling techniques, this protocol offers high specificity and ease of implementation.